Molecular genetic analyses support important roles for the AtGATA2 gene in brassinolide (BR) and light regulation of plant development. The overexpression line 6-9 of AtGATA2 suppresses the etiolated phenotype of Col-0 grown in the dark.
Integration of light- and brassinosteroid-signaling pathways by a GATA transcription factor in Arabidopsis.
Specimen part
View Samples30 collars were taken from wild type plants or antiOsLIC transgenic plants respectively. One collar from one plant only. The leaves are just sprout 2-3 cm (about 1-2 days) from the stem. For measuring the genes expression level, Wild type plants were taken as control. The developing collar from both line2 of OsLIC antisense transgenic plants and wild type were harvested at the heading stage. The position of the collar was about 1cm above the last developed collar. Total RNA was extracted from the collars using TRIzol regeant (Invitrogen, P/N 15596-018, USA) and purified by using Qiagen RNeasy columns (QIAGEN, Cat. NO. 74104). All the processes for cDNA and cRNA synthesis, cRNA fragmentation, hybridization, washing and staining, and scanning, were conducted according to the GeneChip Standard Protocol (Eukaryotic Target Preparation, Affymetrix). Poly-A RNA Control Kit and the One-Cycle cDNA Synthesis kit were used in this experiment as described in the website: http://www.affymetrix.com/products/arrays/specific/rice.affx.
OsLIC, a Novel CCCH-Type Zinc Finger Protein with Transcription Activation, Mediates Rice Architecture via Brassinosteroids Signaling.
No sample metadata fields
View SamplesMTDH was identified to be a functional gene in cancer progression. We overexpressed the gene in MCF7 cells and explored its functions in cancer stem cell maintainance.
No associated publication
Sex, Specimen part, Disease, Cell line
View SamplesInvestigation of differentially expressed genes in circZbtb20 or Nr4a1 deficient ILC3s
No associated publication
No sample metadata fields
View SamplesInvestigation of differentially expressed genes in Ccp6-depleted D3 ESCs
No associated publication
No sample metadata fields
View SamplesInvestigation of differentially expressed genes in Ccp2-deficient CHILPs
IL-7Rα glutamylation and activation of transcription factor Sall3 promote group 3 ILC development.
No sample metadata fields
View SamplesInvestigation of differentially expressed genes in circKcnt2-deficient ILC3s
No associated publication
No sample metadata fields
View SamplesSeven-day-old white-light-grown Arabidopsis seedlings were exposed for 15 minutes to polychromatic radiation with decreasing short-wave cut-off in the UV range, transferred back to the standard growth chamber and samples were taken 1 and 6 hours after the start of irradiation.
Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis.
Age, Time
View SamplesExpression analysis of mature Arabidopsis trichomes in Col-0 and two mutants, triptychon (try-JC) and glabra3 (gl3-3)
Transcriptional profiling of mature Arabidopsis trichomes reveals that NOECK encodes the MIXTA-like transcriptional regulator MYB106.
Specimen part
View SamplesThree independent sets of purified RNA from body cells and great vessel cells of zebrafish embryos were prepared and labelled. Samples were then hybridized onto a Zebrafish AffymetrixGeneChip.
No associated publication
Age, Specimen part
View Samples