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Accession IconSRP154834

RNA-seq profile of expanded human ST2-transduced Tregs cultured with IL-2 and TCR in the presence or absence of IL-33

Organism Icon Homo sapiens
Sample Icon 32 Downloadable Samples
Technology Badge IconNextSeq 500

Submitter Supplied Information

Description
Because of the extensive data in mice supporting the concept that ST2+ Tregs might have desirable therapeutic properties, including tissue repair function, high suppressive capacity, and enhanced stability, we engineered human blood Tregs to constitutively express ST2 (IL-33R). Here we used RNA sequencing to explore the effects of short-term culture with IL-33 on human ST2-transduced Tregs. Overall design: Human naive Tregs flow-sorted from 4 independent donors were lentivirally transduced with ST2, expanded for 13 days, then stimulated with IL-2 and TCR (16 h) or IL-2, TCR, and IL-33 (16 h).
PubMed ID
Total Samples
32
Submitter’s Institution
No associated institution

Samples

Show of 32 Total Samples
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Title
Sex
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R7-D13-16hAug23-9SR-T1N: Donor 2, ST2-transduced Treg + TCR (16h)
female
naive treg
2
NA
R7-D13-16hAug23-9SR-T1N: Donor 2, ST2-transduced Treg + TCR (16h)
female
naive treg
2
NA
R7-D13-16hAug23-9SR-T1N: Donor 2, ST2-transduced Treg + TCR (16h)
female
naive treg
2
NA
R8-D13-16hSep6-4SR-T1N: Donor 3, ST2-transduced Treg + TCR (16h)
female
naive treg
3
NA
R8-D13-16hSep6-4SR-T1N: Donor 3, ST2-transduced Treg + TCR (16h)
female
naive treg
3
NA
R7-D13-16hAug23-3SR-T1n: Donor 1, ST2-transduced Treg + TCR (16h)
male
naive treg
1
NA
R7-D13-16hAug23-9SR-T1-33N: Donor 2, ST2-transduced Treg + TCR + IL-33 (16h)
female
naive treg
2
NA
R8-D13-16hSep6-4SR-T1N: Donor 3, ST2-transduced Treg + TCR (16h)
female
naive treg
3
NA
R8-D13-16hSep6-4SR-T1-33N: Donor 3, ST2-transduced Treg + TCR + IL-33 (16h)
female
naive treg
3
NA
R7-D13-16hAug23-3SR-T1-33N: Donor 1, ST2-transduced Treg + TCR + IL-33 (16h)
male
naive treg
1
NA
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