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Accession IconSRP064177

Transcriptional regulation by Set1 H3K4 methyltransferase and Jhd2 H3K4 demethylase

Organism Icon Saccharomyces cerevisiae
Sample Icon 15 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

Submitter Supplied Information

Description
Histone H3K4 methylation is connected to gene transcription from yeast to humans, but its mechanistic role in transcription and chromatin dynamics remains poorly understood. Here, we investigated the functions for Set1 and Jhd2, the sole H3K4 methyltransferase and H3K4 demethylase, respectively, in S. cerevisiae. Our data show that Set1 and Jhd2 predominantly co-regulate transcription. To further understand the role for H3K4 methylation, we overexpressed Flag epitope-tagged SET1-G990E (a dominant hyperactive allele of SET1) in yeast using the constitutive ADH1 promoter (ADH1p). As a control, we also overexpressed Flag epitope-tagged wild type SET1 in yeast. Analysis of gene expression in set1-null, jhd2-null and wild type SET1 or hypeactive SET1-G990E overexpressing mutants together revealed that the transcriptional regulation at a sub-set of genes, inclduing those governing glycogen metabolism and ribosome biogenesis, is highly sensitive to any change (i.e., loss or gain) in H3K4 methylation levels. Overall, we find combined activities of Set1 and Jhd2 via dynamic modulation of H3K4 methylation contribute to positive or negative transcriptional regulation at shared target genes. Overall design: Gene expression changes were generated from five different yeast strains representing wild type control, set1 null and jhd2 null mutants, and wild type SET1 or dominant hyperacive SET1-G990E overexpressing mutants. Three independent biological samples were grown for each strain, total RNA was isolated, libraries were prepared, sequenced, and analyzed separately.
PubMed ID
Total Samples
15
Submitter’s Institution
No associated institution

Samples

Show of 15 Total Samples
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Additional Metadata
set1_1
ymc64
mid-log phase yeast cells
NA
Set1Flag_1
ymc42
mid-log phase yeast cells
NA
WT_2
htabwt
mid-log phase yeast cells
NA
jhd2_2
ymc70
mid-log phase yeast cells
NA
set1_2
ymc64
mid-log phase yeast cells
NA
Set1Flag_2
ymc42
mid-log phase yeast cells
NA
set1_3
ymc64
mid-log phase yeast cells
NA
Set1G990E_3
ymc43
mid-log phase yeast cells
NA
WT_1
htabwt
mid-log phase yeast cells
NA
jhd2_1
ymc70
mid-log phase yeast cells
NA
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